| 魏永路，任 锐，高 洁，刘伟平，谢 琦，朱根发，杨凤玺.建兰花叶病毒广东分离物的全基因组序列分析[J].广东农业科学,2022,49(2):109-117 |
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建兰花叶病毒广东分离物的全基因组序列分析 |
| Complete Genome Sequence Analysis of Guangdong Isolates of Cymbidium Mosaic Virus |
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| DOI：10.16768/j.issn.1004-874X.2022.02.013 |
| 中文关键词: 兰科植物 病毒检测 建兰花叶病毒（CymMV） 序列分析 选择压力 |
| 英文关键词: orchid virus detection Cymbidium mosaic virus (CymMV) sequence analysis selection pressure |
| 基金项目:广州市科技计划项目（202102020809）；广东省自然科学基金（2017A030312004）；广东省科技计划项目（C2024900075，C2024900210） |
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| 中文摘要: |
| 【目的】建兰花叶病毒 (Cymbidium mosaic virus，CymMV) 是最主要的兰花病毒病原之一，其广泛
传播对兰花产业发展造成严重威胁。探究 CymMV 遗传信息和进化，为广东省兰花病毒病的监测预警和兰花抗病
毒病基因工程提供重要科学依据。【方法】对采自广州地区的墨兰疑似病毒病叶片进行 CymMV 的 RT-PCR 及
DA-SELISA 检测鉴定，利用相关分子生物学软件对 CymMV 分离物进行基因组序列组装、注释、系统进化及选
择压力分析。【结果】首次在广东省发现 2 个 CymMV 分离物 GZV013 和 ZC-29，基因组全长均为 6 227 nt，编
码 5 个功能蛋白；GZV013 与台湾分离物 M2 的核苷酸序列一致性为 97.03%；ZC29 与南京分离物 NJ-1 的核苷酸
序列一致性为 97.11%；CymMV 各分离物的全基因组序列一致性为 86.85%~98.31%，且多样性种群的分化与寄主
种类和地理隔离关系密切；CymMV 基因组每个区域均受到负选择的影响，并符合中性进化模型；编码 RdRp、
TGB1 和 TGB2 的基因在基因组中变异率最高。【结论】GZV013 与台湾分离物 M2 的亲缘关系最近，ZC29 与南
京分离物 NJ -1 的亲缘关系最近，同属于一个分支。 |
| 英文摘要: |
| 【Objective】Cymbidium mosaic virus (CymMV) is one of the most important orchid viruses. The wide
spread of CymMV poses a serious threat to the development of orchid industry. The study is conducted to explore the genetic
information and evolution of CymMV, which will provide an important scientific basis for monitoring and early warning of
orchid virus disease and genetic engineering of orchid virus disease in Guangdong Province. 【Method】RT-PCR and DASELISA were used to detect and identify CymMV from leaves with suspected virus disease of Cymbidium sinense collected
from Guangzhou area. The genome sequence assembly, annotation, phylogeny and selection p ressure analysis of CymMV
isolates were performed with related molecular biology software.【Result】Two CymMV isolates (GZV013 and ZC29) were
found in Guangdong Province for the first time in this study. Both of the genome lengths of GZV013 and ZC29 were 6 227 nt, encoding five functional proteins. The similarity analysis of the full sequence showed that the nucleotide sequence identity
of GZV013 and Taiwan isolate M2 was 97.03% and that of ZC29 and Nanjing isolate NJ-1 was 97.11%. The complete genome
sequence identity among CymMV isolates ranged from 86.85% to 98.31%, and the differentiation of diverse populations was
closely related to host species and geographical isolation. Each region of CymMV genome was affected by negative selection
and conformed to the neutral evolution model. The RdRp, TGB1 and TGB2 genes exhibited the highest genome-wide diversity.
【Conclusion】The GZV013 was most closely related to Taiwan isolate M2, and ZC29 was most closely related to Nanjing
isolate NJ-1, belonging to the same branch of a family. |
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