Tissue culture and rapid propagation technique of four ornamental lily, Lilium Mona, Lilium Manissa, Lilium Barbaresco and Lilium Sorbonne were studied.The results showed that sterilized 10 s by 75% alcohol, treated 10 min by 0.1% mercuric chloride, and then cleaned 5 times by sterile water, formed the best technique of sterilization with the lowest contamination rate. The best induction medium was MS+6-BA 0.5 mg/L+NAA 1.0 mg/L. The best multiplication medium was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L. The best rooting medium was MS+IBA 0.5 mg/L. The best transplantation medium was vermiculite:yellow soil=1:1. The whole set of tissue culture and rapid propagation technique was suitable for the four ornamental lily in this study. |