| Using rapid-amplification of cDNA ends and semi-quantitative RT-PCR technique, the full length of the differentially expressed gene (F64) was obtained and the expression in the rice field eel (Monopterus albus) was conducted at different gonad developmental stage. The results showed that the full length of F64 was 1 721 base pair (bp) that comprises of a 5'-UTR of 142 bp, a 3'
-UTR of 268 bp and an open reading frame (ORF) of 1 311 bp which encoded a 436 amino acid with no signal peptide. Homology analysis suggested that it was a new reporter gene. F64 was not transcription at the early stage of ovary and began to express in ovary at V stage, with the ovary abortive and testis development the transcription level was significantly increased. |
