| In this research, p94 gene with 1 100 bp nucleotide was cloned from a Leizhou black duck by RT-PCR. It encoded a
peptide with 351 amino acids, including a high proportion of hydrophilic amino acid. Through analysis on protein structure, it found that there was no transmembrane and signal peptide, which was similar to chicken’s p94 protein structure. Compared with human, mouse,chicken and other species on sequence identity, Leizhou black duck was 93.8% similar to that of chicken. And in phylogenetic tree,Leizhou black duck and chicken were in the same branch. In a word, it infered that p94 protein function of Leizhou duck was similar to that of chicken which participate in the development of muscle fibers and regulation of muscle tenderness. This provides a theoretical basis for expression and regulation of p94 on meat tenderness. |
