| The aim of this paper was to establish an UV spectrophotometry method for the determination of enrofloxacin content in
enrofloxacin nanoemulsion. A good linearity was obtained for enrofloxacin in the range of 2.0~7.5 ug/mL and the standard curvilinear equation was Y =0.1058X+0.0074 (r=0.9997). The lowest detectable limit concentration was 0.025 ug/mL. The relative standard deviation (RSD) of the precision test was 1.02%, the average recovery rate and its RSD was 99.67(依1.70)% and 1.71%,respectively. The absorbance value was no significant change in 36 h and RSD of the repetitiveness test was 0.75%. The mass concentration of enrofloxacin was 115.50 g/L in the preparation of enrofloxacin nanoemulsion. The precision, recovery rate, stability and repetitiveness were all consistent with the request. The method could be used for the quality control of enrofloxacin nanoemulsion. |
