| On 961 bp mitochondria DNA control region sequences of 44 individuals in 3 Cranoglanis bouderius groups（Nanfeng,
Panjia, Baisha）from the Songtao reservoir in Hainan province, the average content of A, T, G, C were 31.2%, 21.7%, 34.0% and 13.1% respectively. 26 variant sites were found, of which 18 sites were polymorphic and 14 sites were parsimony informative. 11 haplotypes were found in 44 individuals, the haplotype diversity and nucleotide diversity were 0.8594 ±0.0169 and 0.00484 ±0.00313 respectively,presenting high haplotype diversity and low nucleotide diversity phenomenon, of which the nucleotide diversity of Baisha group was highest （0.00517±0.001847）, and Panjia group had the poorest nucleotide diversity (0.00482±0.004522). Tajima 's D (0.36899, P>0.10) and Fu' Fs (0.09041, P>0.10) neutral testing and nucleotide mismatch distribution analysis showed that C. bouderius might not have experienced a large-scale expansion. Fst analysis (-0.00324~-0.01878, P<0.05) and AMOVA analysis (-3.87%, P<0.05) suggested that no obvious genetic differentiation were found among populations, indicating that the 3 groups should be managed as the same population. As far as C. bouderius in Songtao reservoir was concerned, Baisha group should be protected in priority. |
