| Complete sequences of mtDNA Cytochrome b gene (1 140 bp) of 38 individuals of Miichthys miiuy collected in
Lianyungang, Zhoushan and Fuzhou along coastal waters of China were analyzed, of which 27 haplotypes were defined by 39 variable sites and 5 parsimony-informative sites. The global haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.960±0.020 and 0.00271± 0.00030 respectively, which showed high haplotype diversity and low nucleotide diversity. Neutral test of Tajima’s (-2.36010), star-like statistical parsimony cladogram and mismatch distribution revealed a population expansion at about 80 thousand years in Miichthys miiuy. Low genetic differentiated index (Fst=-0.0190~-0.0081) and high gene flow (Nm=-62.53~-26.82), intertwined haplotypes of various geographic populations in Neighbor-joining and TCS tree, and analysis of molecular variance (AMOVA, -1.12%) among populations showed no significant population differentiation,and the three populations could be protected as a single management unit, which were inconsistent with conventional partitions of geographical population. A certern degree of migration in the life history and insufficient time to attain a equilibrium after a large range population expansion may be attributed to account for the lack of genetic structure. |
