| The GRAS proteins are a recently identified family of plant-specific proteins, which play a crucial role in diverse plant
growth and development. Specific primers was designed according to the 5'region of SpSCL1 gene from Sesuvium portulacastrum, the 1 254 bp 5'regulatory sequence of SpSCL1 was cloned from shoot genomic DNA of Sesuvium portulacastrum by Genome Walking method. Sequence analysis showed the sequence contains 681 bp of the promoter regulation sequence and the gene coding region sequence of 573 bp, the promoter regulation sequence consisted of a typical core promoter region of eukaryotic (-60~-10 bp), several promoter element such as TATA-box and CAAT-box, as well as many cis-regulatory elements related to salt, dehydration and stress responses, there are also some cis-regulatory elements related to light responses. It suggested that SpSCL1 gene might play an important role in the salt tolerance, drought tolerance, phytochrome signal transduction pathways. |
