| In this paper, the agglutination reaction for the polysaccharide from leaves of Camellia chrysantha (Hu) Tuyama with
ricinus communis agglutinin I, cristagalli Lectin, euonymus europaeus lectin, maackia amurensis lectin, concanavalin A and peanut lectin, respectively, were discussed by colorimetric method. The results showed that the agglutination reactions for the polysaccharide occurred with all of the lectins, but the reaction degree were remarkable different. The degree of agglutination reaction with ricinus communis agglutinin I was the strongest, where galactose was the specific binding sugar residues. In contrast, the degree of agglutination with cristagalli Lectin, euonymus europaeus lectin, maackia amurensis lectin and peanut lectin, were rather weak, although their specific binding sugar residue were similar. The absorbance of the agglutination reaction of ricinus communis agglutinin I was 6~10 times related to that of the other lectins, while the polysaccharide concentration increasing to 6.59 mg/mL. On the other hand, concanavalin A, mannose
as the specific binding sugar residue, had the similar agglutination reaction character, but the agglutination degree was weaker than that of the ricinus communis agglutinin I. This result implied that the molecular conformations of the lectins affect their affinities with the polysaccharide. |
