| The study mainly discussed the genetic variation of NP gene of H3N2 subtype feline influenza virus after infection of
canine-origin influenza virus. The feline was artificially infected with H3N2 subtypes canine influenza virus. The full length of NP gene was compared with the other 14 different types of influenza viruses in the Genbank datebase. The results revealed that similarity between each other was ranging from 79.3% to 99.7%. The nucleotide identity between infection and inoculation A/canine/Guangdong/1/2007(H3N2) was the highest (99.7%). After the comparison of gene sequence, five mutations were found in the coding region (A33G, A81G,C235T, G345A, A567G). However, only one amino acid mutations was found (I189M). The results showed that canine influenza virus can spread across species, although the variation of NP gene was not significant, but the amino acids mutations caused by the 567 base provide reference statistics for the study of heredity and Species-specific of NP gene. |
