| The prevention of contamination and browning during tissue culture of Nothapodytes nimmoniana was investigated. The
results showed that the lowest contamination rate was achieved with 1-year-old tender stem as explants in March and April, then the contamination rate was 17.5%, browning rate was 36.2% and induction rate was 43.3%. In the 4 parts of stems, the induction effect of semi-lignified stem segments was the best, its induction rate was 51.7%. Low temperature pretreatment of explants had no significant effect on contamination control. The sterilizing effect of “0.1% HgCl2 8 min + 50 mg/L Cefotaxime 30 min” on the stem explants was the optimum. The lowest browning rate of the explants was achieved with the addition of 1.2 g/L activated carbon plus 2.0 g/L polyvinylpyrrolidone (PVP) to the medium, then the browning rate was 25%. |
