The physiological response of Brassica juncea L. seeds in germination to cadmium was studied using Fourier transform
infrared (FTIR) spectrometry with different Cd2+ concentrations (0.0, 0.001, 0.005 and 0.01 mg/mL). The results indicated that, after 14 days stress of Cd, B. juncea L. can grow normally, and no any symptom of Cd toxicity is observed when the Cd concentration is less than 0.001 mg/mL. Compared to the control sample, a lower germination percentage is obtained at the Cd concentration of 0.01 mg/mL. The absorbance of the dominating bands near 1 064, 1 381, 1 643, 2 924, 3 417 cm-1 which corresponded to organic acids, protein amino acids and carbohydrate are initially elevated, and then are decreased in root tissues. With the increase of Cd concentration, the carbohydrate band height of radicle is increased, while a reverse variation trend is obtained for the seedling, indicating that carbohydrate can be transported from seedling to radicle to mitigate the toxic effect of Cd. |