| Ultrafilter was used for extraction of bromelain from crude aqueous extract of pineapple wastes (core and peel). On the
base of single factor experiment, press, temperature and membrane inlet velocity were studied in detail for the comeouts of activity recovery and flux of membrane. According to the Box -Behnken central composite experimental design principle, response surface methodology was adopted to discuss the influence caused by the three facts to target parameters. In addition, the quadratic polynomial regression model was established to optimize the extraction techniques. The result showed that the influence of every single fact to result was significant. And the interaction between temperature and press as well as membrane inlet velocity influenced the activity recovery significantly. The optimized conditions for the extraction were as follows: press 266(±2)kPa, temperature 12(±0.5)℃and membrane inlet velocity 21 (±0.2)mL/s which resulted in activity recovery of 88.45% and flux of membrane of 78 mL/s respectively with relative errors below 2%. The results indicated that the empirical model developed by response surface methodology was adequate to describe the
relationships between the factors and response value. |
