| In this study, an approximately 1 037 bp DNA sequence of bitter taste receptor Tas2R2 gene was obtained by PCR and
cloning technique from genomic DNA sample of Masked palm civet (Paguma larvata), which contained complete single exon (without intron), 915 bp in size, encoding 304 amino acid residues. The pI of the protein is 9.84 and its molecular weight is 34.87 ku. High structure prediction shows this Tas2R2 protein comprises 7 transmembrane regions,4 extracellular regions and 4 intracellular regions, and contains three N-glycosylation sites, three serine phosphorylation sites,three tryptophan phosphorylation sites and one tyrosine site. The Tas2R2 is a hydrophobic protein with less hydrophilic components. The homology analysis of the Tas2R2 gene sequences by Clustal W indicated that the cDNA sequence homologies of Tas2R2 gene of Masked palm civet with cat, dog, giant panda, cow, horse, chimpanzee and mouse is 93.0%、89.4%、89.9%、85.4%、85.9%、84.6%、72.2%, respectively and the homologies of corresponding amino acid sequence is 88.8%、81.1%、83.2%、75.9%、77.8%、75.5%、61.3%, respectively. In addition, phylogenetic tree of Tas2R2 gene from these eight species shows that Masked palm civet is the most close to cat. |
