| The pollens of Melastoma candidum were taken as materials, whose morphology structure was observed under SEM. The best method for the determination of pollen viability was selected, and then the effects of light and storage condition on pollen viability were investigated as well. The results showed that the morphology of pollens from dimorphism stamens appeared no significant difference. They were elliptic from equatorial view and subtrilete spheroidal from polar view. The average polar was 33.04(±1.37) μm, equatorial axis of the pollen was 15.05(±0.88) μm. In vitro culture method was more reliable than TTC staining in determining the pollen viability of M. candidum. The viability of pollens from purple stamens and yellow ones showed no difference. The best culture medium for the germination of the former was
sucrose 50 mg/L + boric acid 5 mg/L + CaCl2 5 mg/L, while the latter one was sucrose 50 mg/L + boric acid 10 mg/L +CaCl2 20 mg/L. Furthermore, the light showed no significant effect on pollen germination. Short-term dormancy appeared in all the three different low temperature storage. Stored at -7益and -23益were better than -4益, whose viability remained at higher rates after stored for 60 days. |
