Flavonoids contained in sweetsop pulp were conformed based on color reaction and UV spectrum with rutin as standard. Using spectrophotometric determination of total flavonoids from sweetsop pulp, extraction process was optimized by Response Surface Methodology. The results showed that the best parameters were that the concentration of alcohol was 51.94%(V/V), the ratio of material and alcohol was l:10 (W/V), the extraction temperature was 81℃and extraction time was 2.5 h. The extraciton volume of flavonoids was 735 mg/100 g. The method is simple, fast, accurate, and practical for extracting the flavonoids in sweetsop pulp. |