| In order to understand the basic characteristics of cassava MeMYB63 gene which relates to drought stress,we cloned its full length cDNA and the 1 500 bp DNA fragment upstream of MeMYB63 gene initiation codon, and preliminarily analyzed the gene promoter activity in transgenic Arabidopsis plants. By using the green fluorescent protein (GFP) MeMYB63 protein was labeled , subcellular localization was operated by means of tobacco transient transformation system to observed fusion protein. And the transcriptional activation function of MeMYB63 protein was confirmed in the yeast transformation experiment. The results showed that the typical promoter elements were found in the 5 ' upstream 1543 bp DNA fragment. MeMYB63 and GFP fusion protein just existed in the nucleus during the subcellular localization experiment of the MeMYB63 protein. Moreover, the result of yeast transcriptional activation experiment showed that MeMYB63 had obvious transcriptional activation function with a transcription activation domain. According to all of the results of the subcellular localization and transcriptional activation, MeMYB63 might be considered as a transcription factor, which provided an important basis for the future study of the gene function. |
