| With marine bass as raw material, we used trypsin prepared active peptides. With degree of hydrolysis as the index,orthogonal experimental for enzymatic hydrolysis conditions were optimized, and used cholic acid salt combining experiments to compare the activity of the active peptides in vitro. The results showed that the optimum enzymolysis conditions for marine bass by trypsin were following: the enzymolysis temperature was 40益, enzyme additives was 20 000 U/g, pH value was 8.5 and the enzymolysis time was 5 h.Under the optimal condition, the degree of hydrolysis reached 12.5%. The result of the cholic acid salt combining experiments showed that the hypolipidemic activity of the active peptides in vitro was related with enzymolysis time. When the enzymolisis time was 5 h, the hypolipidemic activity was the highest, which was closed to cholestyramine. |
