| Shanlan upland rice is one of major types of upland rice, which is of important social, ecosystem and economic value. Inorder to investigate Na+ and K+ transport mechanism of HKT2 in Shanlan upland rice in Hainan,a pair of primers were designed based onthe conserved sequences of the HKT2 genes registered in GenBank from Oryza sativa. Reverse transcription was performed immediately after the extraction of the total RNA from the root, after the amplification of the partial cDNA by PCR. The PCR product was sub-cloned into pMD18-T vector, used PCR detection the sequence. The sequencing results indicated that the length of the amplified cDNA fragment was 1 593 bp and encoding 530 amino acid. Sequence analysis suggested that the nucleotide sequence and the translated amino acid sequence shared over 94豫and 91豫of homology to HKT2 gene sequences from Oryza sativa, respectively. |
