| Buckwheat allergy protein (BW10KD) has a strong reaction to allergic patients' serum IgE. The objectives of this studywere to deposit information on structure and function of BW10KD gene and evolution possibility of different species, and to provide a newmethod for analyzing DNA variation occurred in different species. The conserved sequence of BW10KD gene was amplified in 58 buckwheat entries (Fagopyrum Mill) belonging to 8 species and a variant species. The phylogenic trees were constructed according to sequence blast results. The amplified sequence sharing 99% homology to the known sequence of allergy protein gene. A total of 306arrangement sites were identified in the 58 entries, excluding deletion and insertion loci. Among them, there were 150 invariable (monomorphic) sites and 157 polymorphic (segregating) sites, including 101 parsimony informative sites and 56 singleton variable sites. There were 201 mutations on Eta site in the whole sequence. Based on data of 19 sites with small variation within taxa and large variation among taxa, the phylogenic relationship was clear among different entries. The sequences of BW10KD gene were highly conserved in F. cymosum, F.gracilipes, and F. urophyllum, showing their close relationships. Cultivated common buckwheat was primarily clustered with its wild type, and further grouped with F. zuogongense. Species F. pilus and F. megaspartanium were involved in the same clade, which was continuously clustered with the group containing cultivated tartary buckwheat and its wild type. |
