| The isolation and cultivation of species are the basis of microalgae products exploring and scientific research. The microalgae is susceptible contamination to other microorganism when cultured, and can not be cryopreserved. The microalgae species isolation faced difficulties. This study compared different concentrations of the three pesticides and antibiotics effects on Micractinium pusillum 18A8, Heynigia riparia CE14-2, Platymonas subcordiformis and Phaeodactylum tricornutum. The optimum concentrations of these pesticides and antibiotics for mircoalgae were determined as 25 mg/L for chlorothalonil, 25 mg/L for water synthomycin, 200 mg/L for Lv Bang, 25 mg/L for Ampicillin, 25 mg/L kanamycin and 1.25 mg/L for nysfungin. The fresh water and seawater containing microalgae were collected from nature, cultrued in medium added three appropriate concentrations pesticides and antibiotics, and eventually the other fungi and bacteria were inhibited, the microalgae single species were obtained. |
