| The phyllosphere is a complicated microecological system where colonized by a variety of Microorganism. The epiphytes could improve the dwelling environment and promote the growth of plant through interaction with the host plant. The Pyridoxal Reductase (PLR)isolated from ecosystem of celery leaf, and determine the sources of the PLR with the phenylhydrazine derivative method.Separation and counts of the microorganism separated from celery leaf, determine the changes of microorgenism at the different storage condition, and study on the enzymatic properties of PLR. The results showed that PLR was mainly derived from the fungus of celery leaf surface. The intensity of the PLR activity was fungi>bacteria> actinomycetes at the same bacteria concentration. The enzyme had an optimal temperature and pH at 37益and 7.5. PLR showed a higher catalytic activity of reduction of PL with NADPH. |
