In the present study, a special kind of wild bitter melon seeds produced in Beihai of Guangxi was selected and analyzed.The content of total fatty acid is 38.0% extracted with soxhlet extraction in the seeds. The seed oil was extracted by supercritical CO2 extraction, By using the orthogonal experimental design, the technological parameters affects such as extraction pressure, temperature, time and CO2 flow factors were confirmed.When the technological parameters is used followed: extraction pressure is 37 MPa,extraction temperature is 40益, the rate of CO2 flow is 40 L/h, extraction time is 2 h , a yield ratio bitter melon seed oil is 97.2%. The main constituents of the oil extracted by supercritical CO2 extraction from the seeds of bitter melon was analyzed by GC/MS. The results showed that 79.6% of the oil, were Polyunsaturated fatty acid . Physiological activity of the seed oil of bitter melon may be related with Polyunsaturated fatty acid. |