| In order to obtain T-2 toxin microcapsule feed of shrimp, microcapsule technology was applied. A study was conducted to evaluate the character, embedding rate and utility ratio of T-2 toxin microcapsule feed. Feed were ground and uniformly mixed with 20 ng T-2 toxin in ethanol then blow-dryed out before embedded by ovalbumin. The character, color, grain size, hardness and viscosity of T-2toxin microcapsule feed were observed. Toxic feed was extracted by deionized water, and then T-2 toxin of extraction liquid was detected by LC-MS/MS. The quantity of T-2 toxin ordinary feed in deionized water was 0.654ng, while the quantity of T-2 toxin microcapsule feed in deionized water was 2.661 ng. Embedding rate was calculated basing on the percentage of T-2 toxin in toxic feed after extraction.Microcapsule toxic feed of T-2 toxin put into the environment of shrimp. Obtain T-2 toxin microcapsule feeds, envelop ratio was 96.73%,utility ratio is 1.7 fold compared with normal feed. Comparision between T-2 toxin microcapsule feed and T-2 toxin ordinary feed informed the embedding rate and envelop ratio of T -2 toxin microcapsule feed higher than T -2 toxin ordinary feed. T -2 toxin microcapsule provides fundamental technology for the research on aquaculture contaminated by T-2 toxin. |
