| Real -time Quantitative PCR assay was performed to estimate the relative mRNA expression level of α-amylase in response to gradients of ecological factors including temperature, salinity, food concentration and water depth. Results indicated that as the temperature rose the mRNA level increased with the highest level at 21益and then followed by a decrease within the range of 21~29℃. A similar expression pattern was presented responding to salinity variation. The mRNA level at midrange salinity(27) was significantly higher than both low and high salinities. In elevated food concentration treatments, mRNA level decreased first and then followed by an increase within the range of 8×104~20×104 cells/mL. The water depth had a great effect on the mRNA level. It was significantly higher in deep water (4,5 m) compared with that in shallow water (1,2 and 3 m). |
