| In order to observe dynamic changes of enzyme in the process of seed germination, the authors tested germination rate,amylase, superoxide dismutase(SOD), catalase(CAT) and nitrate reductase activity under 5 kinds of NaCl stress(0, 50, 100, 150, 200 mmol/L)in the rice seed germination. The results showed that in the process of seed germination, germination rate, amylase, SOD, CAT and nitratereductase activity decreased with the increasing of NaCl concentration. Under the NaCl concentration of 150 mmol/L and 200 mmol/L,germination rate was extremely significant lower than that of control group, amplituded by 39%~100%. Under the NaCl concentration of 100 mmol/L and 200 mmol/L, amylase was significant lower than that of the control group, decrease amplitude of 100 mmol/L and 200 mmol/L NaCl treatment were 4.34%~49.13% and -89.35%~119.73%, respectively. SOD activity decreased significantly in the 100 mmol/L NaCl treatment, amplituded by 7.5%~50.19%. CAT activity decreased significantly in 50 mmol/L and 100 mmol/L NaCl treatment, and were significantly different in 150 mmol/L and 200 mmol/L NaCl treatment, amplituded by 1.76%~50.61%. Nitrate reductase activity of 4 salt treatment decreased but not obvious. |
