| Cassava is a high-starch, stress resistance, drought-resistant tropical typical crops. Soluble starch synthase (SSS) is one of the key enzyme in plant starch synthesis process. According to the published SSS郁gene sequences from arabidopsis thaliana, the cassava爷s SSS gene sequences were acquired from AM560 and KU50 genomic DNA sequence database by nucleotide blast and Protein blast. Template specific primers were designed according to the published cassava MeSSScDNA sequence as well as part of the promoter sequence, then cloned about 1 068 bp promoter sequence of MeSSS from cassava cultivar KU50 genomic DNA, the sequence contained 978 bp promoter regulatory sequence and 90 bp coding sequence. Sequence analysis showed that the promoter regulatory sequence contains typical eukaryotic core promoter region, in addition to comprising a plurality of TATA-box, CAAT-box, etc. promoter elements and a variety of dehydration, hormone and light response cis-acting elements, especially important sugar signal cis-elements, such as CGACGOSAMY3, DOFCOREZM, SREATMSD, SURE and so on. These components indicates MeSSS郁expression may have positively relation to the sugar signals. The above results indicate cassava MeSSS郁may be involved in variety of metabolic processes, and its expression may be regulated by a variety of regulatory factors. |
