| The samples collected from Guangdong province were inoculated to Marc-145 cell, the PRRSV specific cytopathogenic effect (CPE) was observed and the isolates named XH-GD obtained. After reverse transcription polymerase chain reaction, the amplified NSP2、ORF3 and ORF5 fragments were analyzed by sequence comparison. The results demonstrated that XH-GD belonged to the North American genotype and its NSP2 had 30 amino acids deletion. The NSP2, ORF3 and ORF5 of XH-GD shared 83.3%~98.9%, 88.6%~99.2% and 88.1%~99.2% of nucleotide similarity, and 76.8%~98.3%, 83.7%~98.8% and 88.1%~99.2% of deduced amino acid similarity,
with that of HUB1, NX06, BJsy06, VR-2332, HB-1 (sh)2002, HB-2 (sh)2002, CH-la and RespPRRS MLV respectively; and shared only 52.9%, 65.0% and 64.3% of nucleotide similarity, and 31.3%, 57.5% and 58.9% of deduced amino acid similarity with that of LV respectively. The phylogenetic tree revealed XH-GD had closer relationship with HUB1, NX06, BJsy06, VR-2332, HB-1(sh)2002, HB-2(sh)2002, CH-la and RespPRRS MLV strains than that of LV strain. |
