| To find specie -specific DNA probes of Fallopia multiflora, the genomic DNA (gDNA) suppression subtraction hybridization (SSH) between Fallopia multiflora and F. multiflora var. ciliinervis was firstly performed. The obtained differential gDNA fragments by SSH were then hybridized with gDNA arrays made of multiple whole genomes of several species (adulterants and/or closely related plants of F. multiflora) and four of the differential fragments were screened unique representing F. multiflora, which can be used as F. multiflora speciss-specific probes. The DNA probes were tested by reserved dot blot hybridization, the results demonstrated the probes could be reliably used to identify F. multiflora. The study of this paper offers the probes representing F. multiflora. The obtained probes are capable to be applied on germplasm or crude drug authentication of F. multiflora and the preparation of Chinese traditional medicines identification gene chip. |
