| To provide much more high quality seeding for farmer, the different factors affect on buds and tissues of Huoshan Kudzu (Pueraria thomsonii Benth) in vitro. The tender branch of Kudzu, include shoots, stems, were used as explants and cultivated in different media. The optimum media for inducing buds, proliferation and rooting were selected by adjusting the kinds and doses of plant hormones and special compounds.The result showed that the tender stem apexes of Pueraria thomsonii Benth was the best materials for inducing tissues. The optimal media to induce sprouting from stems were MS + 6-BA 0.1 mg/L + 2, 4-D 0.1 mg/L. And media MS + 6-BA 0.5
mg/L + 2, 4-D 1.0 mg/L was suitable for induce much more tissues from stems. Otherwise, media MS + 6-BA 0.1 mg/L + 2, 4-D 0.5 mg/L had the abilities to induce sprouting, tissues formation and buds polarization effectually. The media MS + NAA 0.5 mg/L was used for roots inducing, the rooting rate was 73.3%. |
