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| Study on tissue culture of capitulum of Gerbera Dachen |
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| Abstract: |
| The effect of sterilization time, size of capitulum and different phytohormone on differentiation of adventitious buds,multiplication and rooting, with torus as the explant was studied. The results showed that 0.1 percent of corrosive sublimate made a good effect on the “dachen”capitulumn for 8~10 minutes long. When the size of capitulum is under 1.0 cm, the browning and death rate is lower which is 3.1 percent. The best inducing-medium of adventitious buds was 1/2 MS + 6-BA 10.0 mg/L +IAA 0.1 mg/L, which made the highest percent-17.4 percent-of inducing rate. The best proliferating-medium of adventitious buds was MS+KT5.0 mg/L +IAA0.5mg/L with 4~5 multiple. The best rooting medium was obtained with 1/2MS+IAA 0.2~0.5mg/L, or 1/2MS+IBA 0.05~0.5 mg/L, or 1/2MS+NAA 0.01~0.02 mg/L, both the rooting frequency and survival rate were high to 100%. |
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