| PCR-SSCP and DNA direct sequencing technology were used in this study to identify the polymorphisms of CDS 572~980 bp region of DRD1 gene in 90 Cherry Valley duck individuals, the association between SNPs and production traits were also performed subsequently. Results showed that two SNPs of A765T and A912G were found in the region and 3 alleles named A, B and C that resulted in 6 kinds of genotypes included AA, BB, CC, AB, AC and BC respectively were confirmed in this study. The CC genetype and allele C with frequencies of 0.5222 and 0.6667 were predominant genotype and predominant allele respectively. Chi-square test demonstrated that the genotypes distribution of SNP locus in the population deviated significantly from Hardy-Weinberg equilibrium (P<0.01) and the gene showed intermediate polymorphism information. Least square analysis showed that shank length of AB genotype individuals were significantly higher than BB, BC genotype. Moreover, individuals with AB genotype had higher shank circumference than those with BC genotypes. But the others did not reach significant level. This work could lay the experimental foundation for the marker-assisted selection of cherry valley duck. |
