| GAPDH gene full length cDNA was cloned by RACE from leaves of Eleutherococcus senticosus. Some characters involving in cDNA sequence, amino acid sequence, conservative domain and relationship were analyzed by bioinformatics method, and the structure and function of GAPDH gene were deduced. The cDNA of GAPDH gene from E. senticosus was successfully cloned.The full length of GAPDH gene was 1 437 bp containing a 1 023 bp open reading frame (ORF) that encoded a protein with 340 amino-acid residues, the relative molecular mass of GAPDH protein of E.senticosus was 37.070 ku and the isolectric point was 7.71. Without transmembrane domain, GAPDH protein of E.senticosus was located in cytroplasm, and belong to hydrophilic protein. The result has made foundation for analysis the key enzyme gene of express variance of E.senticosus in transcription level. |
