| Qianbei Ma Goat and White Goat of Guizhou were used to construct DNA pools, the complete sequences of extro 2 and part of intron 1 region in TFAM of the two breeds were obtained by designing one pair of specific primers. Then by conducting bidirectional sequencing after purification of PCR production subsequently. DNAStar and BLAST software were used to identify polymorphisms of goat TFAM genes. Moreover, through bioinformatics software to analyze the impact of secondary structure of RNA, secondary structure and tertiary structure of TFAM protein. The results showed that five SNPs (T1005C, G1099C, A1130C, G1164C, T1287C) were found in TFAM which included one missense mutation (G1099C, Gly--Cys), one synonymous mutation (T1005C, Val) and the other SNPs located in intron region respectively. The polymorphisms might lead to secondary structure change of RNA and the structure change of TFAM protein. |
