| In order to find the root-knot specificity promoter of tobacco induced by root-knot nematode and to understand the molecular mechanism of giant cells formation, by using promoterless reporter gene (GUS), in the next report gene Ds transposon, used for the separation special promoter of tobacco root-knot in giant cell. The p13DGUTs transgenic tobacco leaves, transgenic plants were
obtained. The root knot nematode infection in pots containing transgenic tobacco, expression of report gene in root, leaf were detected respectively. The results showed that we screened the special transgenic plant which expressed GUS gene only in giant cells induced by root-knot nematode. |
