| Total DNA of Abrus rnollis and A. cantoniensis in different areas was extracted by modified CTAB, PCR amplification of rbcL was sequenced, MEGE5.1 software was applied to take contrived sequence analysis, genetic distance was calculated, and phylogenetic adjacent tree was eatablished. The results showed that A. rnollis and A. cantoniensis reached a branch each with phylogenetic relationship, indicating rbcL sequence could successfully distinguish the both. Therefore, rbcL sequences can be used as DNA barcode of A. cantoniensis. |
