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| Bioinformatics analysis of Botrytis cinerea BCRho3 gene |
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| Abstract: |
| This research used bioinformatical methods to predict the physical -chemical characteristics, hydropathy and hydrophobicity, signal peptide, subcellular localization, structure and function of BCRho3 gene from Botrytis cinerea, and constructed phylogenetic tree of Rho3, all of this would provide further support for following research. The result shows that BCRho3 is a hydrophilic protein which has not signal peptide and transmembrane structure, its secondary structure is primarily composed of alpha helix and random coil and it performs biological function mainly in the cytoplasm. Sequence analysis indicates that this protein might functions as growth factor, and may involve in transcription regulation and transcription, so it may play important role during growth and development such as spore production, thus it is likely to play a key role in pathogenicity of B.cinerea. The homology and evolutionary tree of Rho3 shows that BCRho3 and related proteins of Sclerotinia sclerotiorum and Marssonina brunnea are close in the phylogenetic tree and identity, which shows highly homology. |
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