|
| Optimization and application of ISSR-PCRreaction system in pear cultivars |
| |
| DOI： |
|
| Hits: 1290 |
| Download times: 620 |
| Abstract: |
| The influencing factors in ISSR reaction system were studied by using genomic DNA of different pear
cultivars. ISSR-PCR optimized reaction systems were eatablished for pear cultivars. The total volume of 25 滋L included
2.5 滋L10伊buffer, 2.5 mmol/L MgCl2, 0.2 mmol/L dNTPs, 0.25 滋mol/L primer, 10~60 ng template DNA and 0.75 U unit of
DNA polymerase. 22 different pear cultivars were used to check the stabilization of the optimized reaction system. The
results showed that the optimal reaction system of ISSR-PCR of pear was stable, repeatable and effective . |
|
View Full Text
View/Add Comment Download reader |
|
|
|
