| To establish appropriate DNA SRAP-PCR amplification system of Conyza blinii Lvl, and lay the foundation
for the molecular markers of C. blinii Lvl. four factors of the silver-stained were optimized, including whether fixed, dye
recipe, silver staining time and composition of developer. This research also explored the SRAP-PCR reaction process and
optimized the amplification system of C. blinii by various factors. The results showed that the annealing temperature of SRAPPCR
reaction program was 51.3, and the optimum SRAP-PCR system (15 L) was as follows: DNA 40 ng, primer 0.7 mol/
L, dNTPs 0.25 mmol/L, Mg2+ 1.9 mmol/L, Taq polymerase 0.6 U, 10buffer 2 L. The program and system were suitable for
silver-stained analysis and were applicable in C. blinii L佴vl. with SRAP marker. |
