| Taking cassava (Manihot esculenta) leaves as the raw material, the optimum extraction conditions and
enzymatic properties of polyphenol oxidase (PPO) from cassava leaves were studied. The results showed that, the optimal
extraction conditions were as follow, pH value of PBS was 6.6, solid-liquid ratio was 4 mL/g and extraction time was 6 h.
The enzymatic reaction of PPO from cassava leaves showed that, the maximum absorption pick was at 399 nm. The
Michaelis constant (Km) was 0.8 mol/L and the enzymatic kinetic equation was V =2000 [S]/0.8+[S] by using catechol as
substrate. The optimal reaction temperature of PPO was 37益and optimal pH value of PPO was 7.0. |
