| To search for new microorganisms with inhibitory activity against plant pathogens, Myoporum bontioides A.
Gray was chosen to be studied. One hundred and four endophytic fungal cultures were isolated from the leaf , leaf vein,
stem, bark, and root samples of Myoporum bontioides A. Gray. They mainly belonged to the genera of sterile mycelium,
Gloeosporium spp., Penicillium spp., Cladosporium spp. and Aspergillus spp. Primary bioassay of their metabolites showed
that about 77.9% strains had inhibitory activity against at least one plant pathogens among Fusarium graminearum,
Gloeosporium musae, Penicillium italicum and Phytophthora sojae (Kaufmann & Gerdemann). And the proportions of the
strains with medium and high activity were ab out 42.3% and 11.5%, respectively. The HPLC analysis showed that the
spectral profiles of the extracts of Myoporum bontioides A. Gray and five selective endophytic fungi with high antifungal
activity were remarkably different, which suggested that these fungi produced different antifungal compounds in contrast to
their mother plant. |
