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| Leaf morphology and genetic diversity of Aquilaria sinensisin Dongguan and its surrounding areas |
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| Abstract: |
| The population genetic diversity and differentiation of Aquilaria sinensis were analyzed by using ISSR. A
total of 105 loci were generated by using 9 primers for 221 samples of 4 A. sinensis population. A toal of 104 loci were
polymorphic and the percentage of polymorphic bands (PPB%) was 99.05%. Observed number of alleles (Na) and effective
number of alleles (Ne) were 1.9905 and 1.5854 respectively. Effective number of alleles (H) and Shannon爷s information
index (I) were 0.3390 and 0.5054 respectively. These results indicated that there was a high level of genetic diversity in A .
sinensis at the species level. Total gene diversity (Ht) was 0.3236 among 4 populations of A. sinensis. Gene diversity within
population (Hs) is 0.2500. The Nei爷s coefficient differentiations (Gst) was 0.2274. The estimate of gene flow (Nm) was
1.6983. The results of UPGMA revealed that the samples of Dianbai population clustered with Dalingshan population
firstly, then clustered with Qingxi population. The samples of Guangxi population didn爷t cluster with the other populations
which showed that it had large genetic divergence form them. The results of UPGMA of individual samples revealed that
between the first and second generations of Dianbai population, there was larger divergence in the large-leaved subgroup
than that of small -leaved one. The small -leaved subgroup of Dalingshan population had close relation with the first
generation subgroup of Dianbai one. The large-leaved subgroup of Dalingshan population had close relation with the Qingxi
population. These results revealed that part of Dalingshan population might come from Qingxi population, and the rest of
Dalingshan population might have the same origins with Dianbai population. |
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