| Granulose cells aer important structural constituent of mammalian ovary, their morphology and function change
following the physiological processes of primordial follicular activation, cell proliferation and differentiation, follicular atresia,
ovulation and luteinization. At the same time, granulose cell is the usual model to analyze cell interaction and signal transduction
of the follicular. Sterile syringes were used to absorb the fluid from the antral follicle. The cells separated and cultured from the
follicular fluid were devoted to the FSHR immune-fluorescence identification, cell cycle detection and vitality test. The results
demonstrated that cells separated from the antral follicle fluid were granules cells, were positive in the FSHR anti -body.
Furthermore, the cell cycle of granulose cell was 24 h, and the most highly cell vitality recched at the 4th day. |
