| An ELISA using conjugated peptides as antigen was established to detect swine antibodies of Foot-and-Mouth
virus Type O. In order to conjugate the peptides to a non-protein carrier, the FMDV peptide sequences selected by bioinformatics,
chemical synthesis and immunity identif ication were done. Using the conjugated peptides as antigen for the indirect ELISA, the
optimal concentration of conjugated peptides, the sera as well as the HRP were detected by phalanx titrations. The critical value
of negative and positive controls, the specificity and sensitivity of this method were confirmed. The immune efficacies of clinical
samples of swine sera were assessed. The results showed that the conjugated peptides as the antigen had effective immunity and
the optimal coating concentration was 1.0 滋g/mL, the optimal dilutions of the positive control serum and the HRP were 1颐32 and
1:8 000, respectively. The method has high specificity and sensitivity. It is easy to operate and saves time. This method could be
used not only to detect sera of FMDV infected pigs, but also to assess the antibody levels of animals immunized by FMDV. |
