| Reverse transcription (RT) PCR was used to amplify the coding sequence of SLA -DQB in liver from
Guangxi Bama mini-pig in order to prepare for SLA-DQB transgenic Guangxi Bama mini-pig. The result showed that the
length of SLA-DQB was 687 bp. 15 missense mutations (Site 10, 14, 19, 27, 29, 38, 39, 48, 58, 61, 68, 72, 76, 78, 183)
were found and led to the change of amino acid in SLA -DQB in Guangxi Bama mini -pig compared with reference
sequence (NM_001113694). Homological analysis showed that there were 95.8%, 95.8%, 88.1%, 86.2%, 80.0% compared
with Sus scrofa, miniature swine, Bos taurus, Homo sapiens, and Mus musculus, respectively. |
