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| Establishment of PCR testing technique forcitrus HLB in Zhaoqing and Yunfu |
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| Abstract: |
| The experiment was made with citrus leaves as samples and by means of DNA extraction. Specific primers
from HLB agents were applied to amplify through PCR, the final product of which would be purified and recollected. 16Sr
DNA segments were positively cloned and sequenced. A PCR testing technique was established for citrus HLB in Zhaoqing
and Yunfu area. The PCR technique was applied to test suspected citrus HLB samples all cross Zhaoqing and Yunfu.
Experiment results indicated that PCR testing technique was feasible and citrus plants with HLB infection were discovered
in Zhaoqing and Yunfu. |
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