| Degenerate primers based on conserved motif of the serine-threonine kinase (STK) region from the cloned
plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of Hainan common
wild rice (Oryza rufipogon Griff). After T/A cloning, sequencing and analyzing by alignment, five RGAs with uninterrupted
open reading frames (ORFs) were obtained. Sequence identity among the five RGAs nucleotide sequences ranged from
17.96% to 98.86%, while the five RGAs deduced amino acid sequences showed identity ranged from 37.82% to 99.25%.
The deduced amino acid sequences of RGAs contained STK motifs and had different homologies to STK resistance genes
that had cloned. These provided the basis for further study on cloning STK resistance gene from Hainan common wild
rice. |
