| Using human DYRK2 cDNA sequence as a probe, screening homology sequences in the EST database, the
DYRK2 gene from cattle was cloned by silico cloning. Sequence analysis showed that cattle DYRK2 cDNA contained an
open reading frame(ORF) with 1 581 bp which encoded 526 amino acids. Protein analysis found that the molecular formula
of encoded protein DYRK2 of cattle was C2632H4204N762O761S26, with weight of 59 532.5 and theoretical pI of 9.53. The result
indicated that the nucleotide sequences of the coding region between cattle, sheep, people and other animals were quite
conservative. |
