| PCR-SSCP was used to detect the single nucleotide polymorphism of GHRL gene of Guizhou Black Goat
and Guizhou White Goat, and its corelation with body weight and body size traits was analyzed. The result showed that a
SNP (C345T) was detected in Exon 4 of GHRL gene, which was a sense mutation and led to two genotypes named CC and
CT. The study of relationships between genotype and body weight and body size traits revealed that the body weight, body
height and chest girth of individuals with genotype CT were very significantly better than genotype CC in Guizhou Black
Goat from Maiping Country. But no significant difference between other indexes and goats(Guizhou Black Goat from Nayong
Country and Guizhou White Goat) was found. The consequence indicated that the SNP of GHRL gene could be regarded as
a candidate molecular marker of MAS affecting growth traits in Guizhou Black Goat. |
