| Several inoculation methods were compared to test their effect of pathogenicity to Stylosanthes guianensias.
In all 1 230 mutants, 23 had decreased weak pathogenicity, 5 even completely losted its ability to infect S. guianensias.
TAIL-PCR was used to identify the T-DNA integration site in the mutant that was coded t-2430 and the sequence of 476
bp was gotten. The flanking sequence of T-DNA was compared with Colletotrichum genome by blast and 401nt of sequence
was found in Contig464 of Colletotrichum genome. By predicting the function of flanking sequence, we found T-DNA
insertion in the promoter region of putative gene, which had 79% homology to Magnaporthe oryzae aspartate
aminotransferase gene (XP_003719674.1). |
